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KMID : 0358419960390010113
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Korean Journal of Obstetrics and Gynecology
1996 Volume.39 No. 1 p.113 ~ p.120
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The Effect of Steroid Hormones on bcl-2 Gene Expression in Rat Uterus
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강성구
김주란/강한승/이병주/김현찬
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Abstract
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The present study aims to determine whether the levels of bel-2 mRNA are changed in endometrium and myometrium of uterus during estrus cycle and ovariectomized rat. For this purpose, we used adult female rats exhibiting at least two consecutive
4-day
estrus cycles and ovariectomized rats treated with oil, progesterone(P4) and estrogen(E2). Endometrium and myometrium tissue were dissected at each stage of estrus cycle and ovriectomized rats treated with steroids and then the total RNAs were
used
to
determine the levels of mRNA using oligo-dT primer. The levels to bel-2 mRNA in endometrium tissue reached a maximum level at estrus stage and the gradually decreased. But in myometrium tissue the levels of bcl-2 mRNA showed a miximum level at
metestrus
stage. Estrogen induced the synthesis of bel-2 mRNA in endometrium and myometrium tissue of ovariectomized rat. These results suggest that there are cyclic fluctuations in proto-oncogene expressionin uterine tissue during estrus cycle and this
may
be
caused by cyclic ovarian steroid secretions.
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KEYWORD
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